Table 2.
The expression profile of ASCs surface markers
| Protein | Name | CD |
|---|---|---|
| Positive expression | ||
| Adhesion molecules | Tetraspan protein | CD9 |
| β-1 integrin | CD29 | |
| Sialomucin | CD34 | |
| α-4 integrin | CD49d | |
| Intercellular adhesion molecule-1 | CD54 (ICAM-1) | |
| Receptor molecules | Hyaluronan receptor | CD44 |
| Transferrin receptor | CD71 | |
| α-Platelet-derived growth factor | CD140a | |
| Enzymes | Neutral endopeptidase | CD10 (CALLA) |
| Aminopeptidase | CD13 | |
| Ecto-5′-nucleotidase | CD73 | |
| Extracellular matrix proteins and glycoproteins | Collagen I | – |
| Collagen III | – | |
| Osteopontin | – | |
| Osteonectin | CD90 | |
| Thy-1 | ||
| Cytoskeletal proteins | Vimentin | – |
| Regulatory proteins of the complement system | Complement decay-accelerating factor | CD55 |
| MAC-inhibitory protein | CD59 | |
| Histocompatibility proteins | HLA-A, -B, -C (class I) | – |
| Negative expression | ||
| Adhesion molecules | Vascular cell adhesion protein 1 | CD106 (VCAM) |
| Lymphocyte function-associated antigen 1 | CD11a | |
| Mac-1a | CD11b | |
| Integrin α-X | CD11c | |
| Platelet endothelial cell adhesion molecule | CD31 | |
| VE-cadherin | CD144 | |
| Receptor molecules | LPS receptor | CD14 |
| Enzymes | Tyrosine phosphatase | CD45 |
| Controversy expression | ||
| Adhesion molecules | Endoglin | CD105 |
| MUC-18 | CD146 | |
| Activated leukocyte cell adhesion molecule | CD166 (ALCAM) | |
| Receptor molecules | Platelet derived growth factor receptor | CD140b |
| Muscle proteins | Smooth muscle actin | – |
| Histocompatibility proteins | HLA-DR | – |
Modified according to Zuk (2013)
Adipose-derives stem cells exhibit specific cell surface expression pattern. Despite intensive search single unique marker have not been identified. ASCs are mainly identified on the basis of their high expression of CD105, CD90, CD44, CD73 in the absence of CD34, CD14 and CD45