Fig. 6.

PARP1 RNAi ameliorates the loss of mitochondrial membrane potential. Changes in ΔΨm between groups of MCs were detected using a multifunctional microplate reader (a) and a fluorescence microscope (b). a The fluorescence signals (Ex = 485 nm/Em = 590 nm) of ΔΨm in MCs were monitored on a multifunctional microplate reader after they were loaded with MitoTracker. b Upon analysis by fluorescence microscopy, the healthy cells present orange florescence (white arrows), and the cells with a decreased ΔΨm emitted only green fluorescence (red arrows). Data are expressed as the mean + SEM (n = 3). **P < 0.01 vs. the control group, ##P < 0.01 vs. the GO group. Scale bar = 50 μm