Fig. 5.

Evolution of the R78-R186 distance and TMD-CTD distance in PIP₂-deleted WT-3SPI⁻ and I223L-3SPH⁻ mutant systems of chicken Kir2.2. The evolutions of the R78-R186 distance (a, b) and TMD-CTD distance (d, e) of the WT (a, d) and I223L mutant (b, e) are presented for each subunit. The corresponding distributions are shown in c and f, respectively. The data of c, f were collected from the tetramer during the last 10 ns of equilibration. References 1 and 2 for the R78-R186 distance (a, b) or TMD-CTD distance (d, e) are the corresponding distances in the crystal structures of the PIP₂-absent (Reference_1) and PIP₂-present (Reference_2) WT and I223L mutant