Table 1.
List of confirmed JAK3 partners in granulosa cells
| Genes | Accession #a | Freq. | Ident. (%) | E-value | Description |
|---|---|---|---|---|---|
| INHBA | NM_174363.2 | 4 | 100 | 0.0 | B.T. Inhibin, beta A |
| LEPROTL1 | BT025351.1 | 2 | 100 | 1e-101 | B.T. Leptin receptor overlapping transcript-like 1 |
| CDKN1B | NM_001100346.1 | 3 | 99 | 3e-146 | B.T. Cyclin-dependent kinase inhibitor 1B (p27, Kip1) |
aAccession number of the best match found following nucleotide sequence comparison via BLAST search in GenBank.Freq., Frequency of cDNA clone identification from yeast two-hybrid prey library; Ident. (%), Identity: represents homology estimates ofbovine prey cDNA fragments with nucleotide sequences in GenBank database; B.T., Bos taurus
Sequences were analyzed for identity and resulted in candidate proteins potentially interacting with JAK3
Plasmids were purified from true positive yeast colonies, amplified by PCR and sequenced