Table 3.
Antibodies used in the expression analyses of Bos taurus proteins by Western blot
| Target proteins | Antibodies | Sources | MW (kDa) Conc. | |
|---|---|---|---|---|
| Beta Actin | Chicken IgY anti-Beta actin antibody | Immune Biosolutions (Cat. # Y00005-002) | 42 | --- |
| JAK3 | Goat polyclonal anti-JAK3 | Santa Cruz Biotechnology (Cat. # sc-1080) | 125 | 0.4 μg/ml |
| STAT3 | Rabbit monoclonal anti-STAT3 | Abcam (Cat. # ab32500) | 92 | 0.069 μg/ml |
| pSTAT3 | Rabbit monoclonal anti-pSTAT3 | Abcam (Cat. # ab32143) | 98 | 1 μg/ml |
| STAT5 | Rabbit polyclonal anti-STAT5 | Abcam (Cat. # ab68465) | 90 | 0.5 μg/ml |
| pSTAT5 | Rabbit polyclonal anti-pSTAT5 | Abcam (Cat. # ab97734) | 90 | 1 μg/ml |
| LEPROTL1 | Rabbit polyclonal anti-LEPROTL1 | Abcam (Cat. # ab139564) | 14 | 1 μg/ml |
| INHBA | Rabbit polyclonal anti-INHBA | Aviva Systems Biology (# ARP54663_P050) | 44 | 0.5 μg/ml |
| CDKN1B | Goat polyclonal anti-CDKN1B/KIP1 | Aviva Systems Biology (# OAEB01630) | 26 | 0.1 μg/ml |
Abbreviations: pSTAT, phospho STAT, MW molecular weight of target proteins analyzed, Conc., antibody concentrations used for immunoblotting