Table 1.
Primers used for reverse transcription and amplification of genes related to liver cancer stem cells
| Gene | Primer sequence(5′→3′) |
|---|---|
| miR-21 | F: ACTCAGCTGG TAGCTTATCAGACTGATG |
| R: TGGTGTCGTGGAGTCG | |
| U6 | F: CTCGCTTCGGCAGCACA |
| R: AACGCTTCACGAATTTGCGT | |
| CD13 | F: TTCAACATCACGCTTATCCACC |
| R: AGTCGAACTCACTGACAATGAAG | |
| Ep-CAM | F: ATAACCTGCTCTGAGCGAGTG |
| R: TGCAGTCCGCAAACTTTTACTA | |
| CD90 | F:ATGAAGGTCCTCTACTTATCCGC |
| R: GCACTGTGACGTTCTGGGA | |
| OCT4 | F: GTGTTCAGCCAAAAGACCATCT |
| R: GGCCTGCATGAGGGTTTCT | |
| GAPDH | F: CTGGGCTACACTGAGCACC |
| R: AAGTGGTCGTTGAGGGCAATG |
F forward, R reverse