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. 2016 Oct 16;2016:2476842. doi: 10.1155/2016/2476842

Figure 1.

Figure 1

Effects of different PTEN phenotypes on A549 cell proliferation, apoptosis, and cell cycle progression. A549 cells were transfected with pGFP (null), pGFP-PTEN (PTEN-wt), pGFP-phosphatase-dead PTEN (PTEN-C142A), or PTEN-siRNA in the presence or absence of LY294002 (10 μM) (PTEN-siRNA and PTEN-siRNA+LY294002). Untransfected A549 cells were used as the control group (con) and these cells were also treated with LY294002 (10 μM) (LY294002). Cell viability (a), the cellular apoptosis rate (b), and cell cycle progression (c) were measured by MTT and flow cytometry, respectively (∗ p > 0.05, ∗∗ p < 0.05, △ p < 0.05, # p < 0.05, □ p > 0.05, and ∘ p < 0.05).