(a).

Reference	Country	Animal strain	Animal number	Sex	Age	Weight	Experimental groups	Animal number per group	Numbers of animals per box	Treatment control group	Plant species	Native/exotic	Used parts	Fractions	Dose	Popular indication	Wound area	Measurement interval	Wound area calculation	Treatment time
Bastos et al., 2011 [15]	Brazil	Wistar rats	12	♂	4 mo	300–320 g	4	3	1	Miconazole and nonionic cream	Piper hayneanum (Piperaceae)	?	L	[CHCl3-EtOAc 1 : 1 (haulm, A) and CHCl3-MeOH 1 : 1 (routs, B)	?	Anti-inflammatory, infectious skin diseases and healing, wounds, hematoma, and ecchymosis	6 mm2	Daily	?	15 days
Shukla et al., 1999 [16]	India	Sprague Dawley rats	?	♂	?	200–220 g	3	?	1	Saline solution	Centella asiatica (Apiaceae)	?	?	Asiaticoside	20 μL of 0.2%	Healing activity	8 mm2	7/7 days	?	14 days
Muralidhar et al., 2011 [17]	India	Wistar rats	42	?	?	150–200 g	7	6	?	Ointment base	Butea monosperma (Fabaceae)	N	Sb	PETFR: petroleum ether fraction BENFR: benzene fraction, CHLFR: chloroform fraction, and ACEFR: acetone fraction	200 mg/kg-	Antitumor, antiulcer, antifungal, and antidiarrheal activities	500 mm2	4/4 days	?	16 days
Süntar et al., 2013 [18]	Turkey	Sprague Dawley rats	?	♂	?	160–180 g	?	6	?	Ointment base	Helichrysum graveolens (Asteraceae)	N	F	Hg-hexane; Hg-CH2Cl2; Hg-EtOAc, Hg-BuOH; Hg-R-H2O; Hg-Fr.B1; Hg-Fr.B2, Hg-Fr.B3, Hg-Fr.A, Hg-Fr.B; and Hg-Fr.C	0.5 g	Antimicrobial, antioxidant, anti-inflammatory, sedative, antidiabetic, and cytotoxic activities	5 mm2	Daily	Reduction in wounded area, using AutoCAD program	12 days
Mekonnen, et al., 2013 [19]	Ethiopia	Swiss mice/Wistar rats	24	?	8–10 wk/3–5 mo	30–40 g/180–200 g	4	6	1	Sodium carboxyl methyl cellulose xerogel and nitrofurazone	Kalanchoe petitiana (Crassulaceae)	N	L	Methanolic and chloroform fractions were 16%, 8.76%, 7.5%, and 5.6%, respectively	?	Wound healing, hemorrhoids, and antibacterial activities	312 mm2	Daily	% wound contraction = wound area on day 0 − wound area on day n/wound area on day 0 × 100	10 days
Pieters et al., 1995 [20]	Belgium	Wistar rats	?	♀	?	250–300 g	20	2	1	Not treated	Croton spp. (Euphorbiaceae)	E	Lx	Polyphenolic: PEG ointment, PEG 400 10%	0,5 mL/2x day	Wound healing	3 cm	Daily	?	18 days
Korkina et al., 2007 [21]	Italy	Wistar rats	40	♂	?	350–400 g	5	10	?	Saline solution	Syringa vulgaris (Oleaceae)	N	F	Two phenylpropanoid glycosides: verbascoside and teupolioside	100 μL (0.2 mg/mL)	Wound healing, anti-inflammatory, antirheumatic, antipyretic, and antifungal activities	2.25 cm2	4/4 days	The recorded wounds were measured by planimetry using special computer program	8 days
Bigoniya et al., 2013 [22]	India	Wistar rats	30	♂	?	175 ± 10 g	5	6	?	Vehicle (not related)	Euphorbia hirta (Euphorbiaceae)	N	Wp	Flavonoid fraction (EHTF)	?	Antimicrobial, antifungal, antiviral, anti-inflammatory, antiarthritic, and antioxidant activities	500 mm2	4/4 days	?	20 days
Lodhi et al., 2011 [23]	India	Wistar rats	30	♂♀	?	150–200 g	5	6	1	Not treated	Martynia annua (Martyniaceae)	N	L	M. annua fraction: MAF-A, MAF-B, and MAF-C	?	?	500 mm2	2/2 days	% Wound contraction = healed area/total wound area × 100	20 days
Tabandeh et al., 2013 [24]	Iran	Wistar rats	60	♂	?	200 ± 50 g	4	15	?	Saline solution	Silybum marianum (Asteraceae)	E	?	Flavonoid silibinin (SB)	10% and 20% SB powder	Hepatoprotective and liver regenerating activities	1 cm	Daily	?	30 days
Sonmez et al., 2015 [25]	Turkey	Wistar rats	24	♂	?	180–260 g	3	8	?	Saline solution	Solanum tuberosum (Solanaceae)	?	?	Polysaccharide hemostat (APH)	3 mg of wheat meal in group 2 and 3 mg of APH in powder form	?	2 × 2 × 2 cm	3, 7, and 14 days	Percentage of contraction = [100 − (total wound area on the 14th day/total wound area on the 3rd day) × 100]	14 days
Karakaş et al., 2012 [26]	Turkey	Wistar rats	12	♂	?	200–250 g	2	12 and 8	?	Not treated	Bellis perennis (Asteraceae)	E	F	n-Butanol fraction	?	Activities in sore throat, headache, eczema, skin boils, and gastritis	4 mm2	1, 5, 10, and 30 days	Percentage of wound area = wound area in day/wound area in the first day × 100; percentage of wound healing = 100 − percentage of wound area	30 days
Choi et al., 2001 [27]	Korea	Hairless mice	10	♂	?	?	2	10	?	Vehicle (not related)	Aloe vera (Liliaceae)	?	?	Glycoprotein fraction named G1G1M1DI2	10 mg/g ointment Gentamicin 0.1%, every day	Wound healing, thermal injury healing, anti-inflammation, and immunomodulation activities	154 mm2	Daily	?	8 days
Parente et al., 2011 [28]	Brazil	Wistar rats	36	♀	60 days	160–190 g	2	18 and 6	1	Distilled water	Calendula officinalis (Asteraceae)	E	F	DCF: dichloromethane fraction at 1%; HCF: hexane fraction at 1%	?	Anti-inflammatory, first-degree burns, and skin rashes activities	1 cm	4, 7, and 14 days	?	14 days
Olugbuyiro et al., 2010 [29]	Nigeria	Wistar rats	16	♂	?	250–300 g	2	4	1	Gentamicin and saline solution	Flabellaria paniculata (Malpighiaceae)	N	L	Chloroform fraction and aqueous fraction	100 mg/mL	Activities in skin infections, wounds and sores, and dysentery	2 × 2 cm	7, 12, 14, and 18 days	?	18 days
Süntar et al., 2010 [30]	Turkey	Sprague-Dawley rats/Swiss mice	?	♂	?	160–180 g/20–25 g	9	6	?	Not treated	Sambucus ebulus (Caprifoliaceae)	N	L	Polyamide column fractions from the methanolic extract (Fr A, B, C, D, and E)	0,5 g	Hemorrhoids, rheumatic pain, treating burns, infectious wounds, edema, eczema, urticarial, and inflammations	5 mm2	2/2 days	Wound contraction was calculated as percentage of the reduction in wounded area	12 days
Kim et al., 2013 [31]	Korea	Hairless mice	10	♀	2 mo	?	2	5	?	Matrigel solution	Panax ginseng (Araliaceae)	?	L	Ginsenoside Rd	10 mL	Strengthening immune system and atherosclerosis activities	?	3/3 days	?	9 days
Chaudhari et al., 2006 [32]	India	Wistar rats	30	♂♀	?	180–250 g	5	6	?	Soft paraffin (85%), cetostearyl alcohol (5%), hard paraffin (5%), and wool fat (5%)	Terminalia arjuna (Combretaceae)	N	Sb	Fraction I hydroalcohol Fraction II phytoconstituents extraction of tannins Fraction III consisted of saponins	0.5 g	Diuretic, cooling, aphrodisiac, expectorant, antidysenteric, urinary astringent, antioxidant, and antibacterial activities	4 cm2	2/2 days	?	16 days
Swamy et al., 2006 [33]	India	Wistar rats	24	♂♀	?	150–200 g	4	6	?	Framycetin ointment	Embelia ribes (Myrsinaceae)	?	L	Embelin	4 mg/mL of 0.2% sodium alginate gel	Anti-inflammatory to relieve rheumatism and fever activities	500 mm2	4/4 days	?	16 days
Hernandes et al., 2010 [34]	Brazil	Wistar rats	15	♂	?	180–200 g	3	5	1	Ointment base	Stryphnodendron adstringens (Fabaceae)	?	Sb	EtOAc fraction	?	Antioxidant, cicatrizant, and anti-inflammatory activities	7 mm2	4, 7, and 10 days	?	10 days
Sidhu et al., 1999 [35]	USA and India	Sprague Dawley rats	?	♂	?	250–300 g	4	?	1	Vehicle PBS	Arnebia nobilis (Boraginaceae)	N	?	Arnebin-1 (5,8-dihydroxy-2-(19-b,b-dimethylaryoxy-49- methylpent-3-enyl)-1,4-naphthoquinone	?	?	8 mm2	Daily	?	11 days
Paramesha et al., 2015 [36]	India	Wistar rats	18	?	?	150–200 g	3	6	?	Sodium alginate	Carthamus tinctorius (Asteraceae)	N	L	Dehydroabietylamine of C. tinctorius L., var. Annigeri-2	50 g to get 0.2% (w/w) ointment gel	Laxative, appetizer, and diuretic also useful in urorrhea and ophthalmopathy activities	?	4/4 days	?	16 days
Nagappan et al., 2012 [37]	Malaysia	Sprague Dawley rats	84	♀	?	200–250 g	7	12	1	Not treated	Murraya koenigii (Rutaceae)	N	L	Carbazole alkaloids mahanine (1) (0.40%) (C23H25NO2), mahanimbicine (2) (0.24%) (C23H25NO), and mahanimbine (3) (0.66%)	Mahanine (1) (0.40%), (2) (0.24%), and (3) (0.66%) (w/w)	Stimulants, tonics, treating influenza, fever, and bronchial asthma activities	8 mm2	Daily	% of wound contraction = Ø of wound area −  Ø of unhealed w.a./diameter of w.a. (wound area) × 100%	18 days
Qu et al., 2013 [38]	China	Sprague Dawley rats	54	♂	?	200–220 g	9	6	1	Vaseline	Amorpha fruticosa (Fabaceae)	N	Fr	6a,12a–dehydroamorphin, D–3–O–methyl–chiro–inositol, Kaempferol-3-gluco- 7-rhamnoside,7,2′,4′,5′-tetrom–ethoxyoflavone, dehydrosermundone, tephrosin, 7,4′-dimethoxyisoflavone	?	?	500 mm2	2/2 days	Percent wound contraction = (original wound area − unhealed area)/original wound area × 100%	22 days