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. 2016 Oct 28;36(22):2855–2866. doi: 10.1128/MCB.00193-16

FIG 3.

FIG 3

ATXN7L3 and ATXN7L3B affect global levels and subcellular distributions of H2Bub1, ENY2, and USP22. (A) Whole-cell lysates from 293T cells stably expressing pINTO-N-FH vector, pINTO-N-FH-ATXN7L3, or pINTO-N-FH-ATXN7L3B were resolved by SDS-PAGE. Proteins were transferred onto membranes and detected by immunoblotting with the indicated antibodies. Arrowheads indicate endogenous (en-) and exogenous (FH-) ATXN7L3 proteins. (B) Cytoplasmic (Cyto) and nuclear (Nuc) fractions were isolated from 293T cells stably expressing pINTO-N-FH vector, pINTO-N-FH-ATXN7L3, or pINTO-N-FH-ATXN7L3B. Proteins were transferred onto membranes and detected by immunoblotting with the indicated antibodies. Arrowheads indicate endogenous and exogenous ATXN7L3 proteins. (C and D) Effects of overexpression of FH-ATXN7L3 or FH-ATXN7L3B on the levels of the indicated proteins occur posttranscription. RNAs were extracted from 293T cells stably expressing pINTO-N-FH vector, pINTO-N-FH-ATXN7L3, or pINTO-N-FH-ATXN7L3B and used as the templates for cDNA generation and quantitative real-time PCR. The mRNA levels of ATXN7L3 and ATXN7L3B (C) and ENY2 and USP22 (D) were examined.