Food and water intake during light and dark cycles. Food intake (A) and water intake (B) in 6-, 10-, and 18- to 20-week-old Kiss1r KO and control females. For the cohort of 18- to 20-week OVX mice, both genotypes were ovariectomized at 2.5 weeks of age. The gonad-intact 18- to 20-week-old food intake graph is reused with permission from our previous publication (14) and is shown for comparison purposes to the other ages; n = 6–9 per group; **, P < .01; ***, P < .001; #, nonsignificant trend for genotype difference (P < .07).