Figure 3.

CLAMS cage assessments of gas exchange and energy expenditure in light and dark cycles. VO₂ (A), VCO₂ (B), and energy expenditure (C) in 6-, 10-, and 18- to 20-week-old Kiss1r KO and control females; n = 7–9 per group. For the cohort of 18-to 20-week OVX mice, both genotypes were ovariectomized at 2.5 weeks of age and remained without gonadal sex steroids for the rest of the study. The gonad-intact 18- to 20-week-old CLAMS data (third column of graphs) is reused with permission from our previous publication (14) and is shown for comparison purposes to the other ages; *, P < .05; **, P < .01; ***, P < .001.