Figure 1.

Foxo1 is efficiently deleted from the pituitary gland in Foxo1^Δpit embryos. Immunohistochemical staining for FOXO1 was performed on coronal sections from Foxo1^Δpit embryos and wild-type littermate controls at e16.5 (A and B) and e18.5 (C and D) and P3 (E and F). FOXO1 (green)-immunopositive cells are almost completely absent in Foxo1^Δpit pituitaries at all ages examined. Images shown are representative of embryos from three different litters for each genotype at each age. Nuclei are stained with DAPI (blue). Scale bars, 100 μm. RT-qPCR also shows the expression of Foxo1 is significantly reduced at e16.5, e18.5, and P3 (G). Values are set relative to Actb for each sample. Data are expressed as mean ± SEM of five animals at each age for each genotype. The data were analyzed by a two-way ANOVA with a Sidak's multiple comparisons test to determine significant differences between Foxo1^Δpit embryos and wild-type littermate controls at each age. **, P < .01).