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. 2016 Sep 15;157(11):4351–4363. doi: 10.1210/en.2016-1372

Figure 3.

Figure 3.

The transition from uncommitted progenitor to committed progenitor occurs normally in the absence of FOXO1. RT-qPCR was performed for Sox2, a marker of uncommitted progenitor cells (A), and Pou1f1, a marker of cells committed to the somatotrope lineage (B) in Foxo1Δpit embryos and wild-type littermate controls. Values are relative to wild-type controls at e16.5. Data are expressed as mean ± SEM of 16 (e16.5) and nine (e18.5) embryos for Sox2 and nine (e16.5) and nine (e18.5) embryos for each genotype for Pou1f1. The data were analyzed by a two-way ANOVA to determine significant differences between Foxo1Δpit embryos and wild-type littermate controls at e16.5 and e18.5. Immunohistochemistry for POU1F1 (green) was performed on coronal sections from Foxo1Δpit embryos and wild-type littermate controls at e16.5 (C and D) and e18.5 (E and F). All cell nuclei are labeled with DAPI (blue). Images shown are representative of sections from four different litters for each genotype at each age. Scale bars. 100 μm.