Figure 7.
FOXO1 is important for the normal expression of Lhb. Immunohistochemistry for LHB (red) was performed on three different litters at e18.5 (A and B). Nuclei are stained with DAPI (blue). Scale bars, 100 μm. C, Area of LHB staining was measured on at least three different slides from three different litters for both Foxo1Δpit embryos and wild-type littermate controls using Image J (National Institutes of Health). Data are expressed as mean ± SEM. The data were analyzed by a Student's t test to assess significant differences between Foxo1Δpit embryos and wild-type littermate controls. D, RT-qPCR was performed for Lhb using total RNA from Foxo1Δpit embryos and wild-type littermate controls at e18.5. Values are relative to e18.5 wild-type controls. The data were analyzed by a Student's t test to determine significant differences between Foxo1Δpit embryos and wild-type littermate controls. *, P < .05.