Table 1.
Protein Target | Antigen Sequence (if Known) | Name of Antibody | Manufacturer, Catalog Number, and/or Name of Individual Providing the Antibody | Species Raised (Monoclonal or Polyclonal) | Dilution Used |
---|---|---|---|---|---|
Phospho-ERK1/ERK2 | p-ERK | Cell Signaling, number 4370 | Rabbit; monoclonal | 1:200 | |
Phospho-SAPK/JNK | p-JNK | Cell Signaling, number 9255 | Mouse; monoclonal | 1:400 | |
Rat ACTH | ACTH | National Hormone and Peptide Program | Rabbit; polyclonal | 1:500 | |
Rat TSHB | TSHB | National Hormone and Peptide Program | Rabbit; polyclonal | 1:2000 | |
Mouse GH | GH | National Hormone and Peptide Program | Rabbit; polyclonal | 1:10 000 | |
Rat LHB | LHB | National Hormone and Peptide Program | Guinea pig; polyclonal | 1:500 | |
Mouse PRL, recombinant | PRL | National Hormone and Peptide Program | Rabbit; polyclonal | 1:10 000 | |
C terminus of human FOXO1 | FOXO1 | Cell Signaling, number 2880 | Rabbit; monoclonal | 1:50 | |
POU1F1 | PIT1 | Simon Rhodes, lot 1603 | Rabbit; polyclonal | 1:2000 |
Abbreviations: JNK, c-Jun N-terminal kinase; SAPK, stress-activated protein kinase.