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Coomassie-stained SDS-PAGE (4 to 12% Bis-Tris, morpholineethanesulfonic acid running buffer) of PF 1 and 2 after IEX and GF. Lane 1, molecular mass marker (BenchMark; Invitrogen); lane 2, PF 1 after IEX; lanes 3 and 4, PF 1 after GF; lane 5, PF 2 after IEX; lanes 6 and 7, PF 2 after GF.
