Position of G. trabeum LPMOs in the phylogenetic tree of LPMO9s. The phylogenetic tree was created from catalytic domains of LPMO9s by using the neighbor-joining method in ClustalX (version 2.1). Several LPMOs are labeled, and their cleavage specificities are indicated (C-1, C-4, or both [C-1/C-4]; N. D., not determined). For all labeled non-GtLPMOs, activity on cellulose has been demonstrated. An asterisk indicates that additional activity on xyloglucan has been detected. The functional data for GtLPMO9A-2 stem from the present report; other functional data come from references 13, 14, 16, 49, and 65).