TABLE 2.
Oligonucleotide primers used in this study
| Primer name | Sequence (5′–3′)a | Note | Restriction site | Reference or source |
|---|---|---|---|---|
| tpnRL17-1 | AACAAGCCAGGGATGTAACG | For sequencing of transposon insertion | Larsen et al. (21) | |
| tpnRL13-2 | CAGCAACACCTTCTTCACGA | Larsen et al. (21) | ||
| AceA_FEco | GCGAATTCACGTGCTCGACTAAGCCTTC | For in-frame deletion of aceA in TX1 | EcoRI | This study |
| AceA_RXho | GCCTCGAGTGCGGACATGGCCAATCCTTC | XhoI | This study | |
| AceA_FXho | GCCTCGAGTTCCACTAAGAACTGGCTCAC | XhoI | This study | |
| AceA_RHind | GCAAGCTTCTCTTGATCAGCGGCAGTTT | HindIII | This study | |
| AceB_FEco | GCGAATTCGAAGCGAAGAACGGGTACAG | For in-frame deletion of aceB in TX1 | EcoRI | This study |
| AceB_RXho | GCCTCGAGTTCAGTCATTGCTTGCCTCAC | XhoI | This study | |
| AceB_FXho | GCCTCGAGGGTCTGTAAGCACCTCGCGCC | XhoI | This study | |
| AceB_RHind | GCAAGCTTAACCGTCTGCAGTCTTTCTGA | HindIII | This study | |
| FixC_FEco | GCGAATTCCAGTGATCCGTCGAAGGTG | For in-frame deletion of fixC in TX1 | EcoRI | This study |
| FixC_RSma | GCCCCGGGGGCGGGCATCAGAAGGGCTCC | SmaI | This study | |
| FixC_FSma | GCCCCGGGGCCAGCTGAATAGGACAAGG | SmaI | This study | |
| FixC_RHind | GCAAGCTTCAGTTCAGCTCCACCTCGAT | HindIII | This study | |
| GlcE_FEco | GCGAATTCAGTTTCGATTCGGTGGAAAA | For in-frame deletion of glcE in TX1 | EcoRI | This study |
| GlcE_RXho | GCCTCGAGATCGGCCATCAGAAACGCTCC | SmaI | This study | |
| GlcE_FXho | GCCTCGAGGAGCTCTGATGCAAACCAACC | SmaI | This study | |
| GlcE_RHind | GCAAGCTTCGTCTGCACTATGGCTTCG | HindIII | This study | |
| aceA(f)_F | GCGAATTCACGTGCTCGACTAAGCCTTC | For construction of pBaceA | EcoRI | This study |
| aceA(f)_R | GCAAGCTTCTCTTGATCAGCGGCAGTTT | HindIII | This study | |
| aceB(f)_F | GCGAATTCGAAGCGAAGAACGGGTACAG | For construction of pBaceB | EcoRI | This study |
| aceB(f)_R | GCAAGCTTAACCGTCTGCAGTCTTTCTGA | HindIII | This study | |
| glcE(f)_F | TGCCCGGGGAGCTGGAACGCGCCG | For construction of pBglcE | SmaI | This study |
| glcE(f)_R | GCACAAGCTTGATCAGGTAGATGCGCC | HindIII | This study |
a
Restriction enzyme sites are underlined.