TABLE 4.
Growth of TX1 mutants on various carbon sources
| Straina | Description | Generation time (mean ± SD) (h)b |
||||||
|---|---|---|---|---|---|---|---|---|
| OPEOn | NPEOn | AEO8 | Ethanol | Acetate | Pyruvate | Glycolate | ||
| TX1 | Wild type | 1.5 ± 0.1 | 3.2 ± 0.1 | 3.7 ± 0.2 | 4.8 ± 0.2 | 2.7 ± 0.2 | 3.6 ± 0.4 | 11.3 ± 3.1 |
| TX1 ΔaceA | Deletion of isocitrate lyase gene | − | − | − | − | − | 3.6 ± 0.4 | 11.5 ± 2.5 |
| TX1 ΔaceB | Deletion of malate synthase gene | − | − | − | − | − | 3.6 ± 0.4 | − |
| TX1 ΔfixC | Deletion of dehydrogenase gene | 1.5 ± 0.1 | 3.1 ± 0.1 | 3.7 ± 0.1 | 4.8 ± 0.2 | 2.7 ± 0.2 | 3.6 ± 0.4 | 10.8 ± 2.2 |
| TX1 ΔglcE | Deletion of glycolate dehydrogenase gene | 1.5 ± 0.1 | 3.2 ± 0.1 | 3.7 ± 0.1 | 4.7 ± 0.1 | 2.9 ± 0.3 | 4 ± 0.2 | − |
| C48-18 | Mutation at diadenosine tetraphosphate hydrolase gene | − | − | − | 4.7 ± 0.2 | 2.7 ± 0.2 | 4 ± 0.2 | 11.2 ± 4.2 |
| C73-29 | Mutation at demethoxyubiquinone hydroxylase gene | − | − | − | 4.7 ± 0.2 | 2.7 ± 0.2 | 4 ± 0.2 | 11.3 ± 3.3 |
| C82-3 | Mutation at glycosyltransferase gene | − | − | − | 4.7 ± 0.2 | 2.9 ± 0.3 | 4 ± 0.2 | 11.5 ± 2.8 |
| C85-47 | Mutation at UDP-N-acetylglucosamine-1-phosphate transferase gene | − | − | − | 4.7 ± 0.1 | 2.7 ± 0.2 | 4 ± 0.2 | 11.7 ± 2.5 |
| B2-48 | Mutation at putative Zn-dependent protease gene | − | − | − | 4.8 ± 0.2 | 2.9 ± 0.3 | 4 ± 0.2 | 10.9 ± 3.2 |
| C3-45 | Mutation at gene encoding membrane protein | − | − | − | 4.7 ± 0.2 | 2.9 ± 0.3 | 4 ± 0.2 | 11.6 ± 2.7 |
| B3-46 | Mutation at gene encoding transcriptional regulator | − | − | − | 4.8 ± 0.1 | 2.9 ± 0.3 | 4 ± 0.2 | 11.8 ± 2.8 |
| C11-3 | Mutation at gene encoding unknown protein | − | − | − | 4.7 ± 0.1 | 2.9 ± 0.3 | 4 ± 0.2 | 11.7 ± 3.1 |
| TX1 ΔaceA(pBaceA) | Complementation of isocitrate lyase gene | 1.5 ± 0.1 | 3.2 ± 0.1 | 3.6 ± 0.2 | 4.7 ± 0.1 | 2.9 ± 0.3 | 4 ± 0.2 | 11.1 ± 3.2 |
| TX1 ΔaceB(pBaceB) | Complementation of malate synthase gene | 1.5 ± 0.1 | 3.1 ± 0.1 | 3.7 ± 0.1 | 4.7 ± 0.2 | 2.9 ± 0.3 | 4 ± 0.2 | 10.8 ± 2.6 |
| TX1 ΔglcE(pBglcE) | Complementation of glycolate dehydrogenase gene | 1.5 ± 0.1 | 3.1 ± 0.1 | 3.7 ± 0.1 | 4.8 ± 0.2 | 2.9 ± 0.3 | 4 ± 0.2 | 11.2 ± 2.8 |
a
Each strain was cultured in 5 ml of MSB plus 0.1% of the relevant carbon source.
b
−, no growth; OPEOn, Triton X-100; NPEOn, Triton N-101; AEO8, dodecyl octaethoxylate. Data are the results from three independent experiments.