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SDS-PAGE of purified LcpK30 muteins. Molecular mass standards (M) with kilodalton values indicated are shown in the left-most and right-most lanes. The type of mutein is indicated in the top line. All proteins had been stored frozen (−20°C) before electrophoresis. For the T168A mutein, the protein band appeared inhomogeneous. This could be attributed to the storage period and storage condition. The freshly purified T168A mutein appeared homogeneous. Wt, wild type.
