FIG 5.
UV-vis spectra of LcpK30 wild type (A) and the R164A mutein (B). UV-vis spectra of purified Lcp in the as-isolated state (black line) and in the as-isolated state to which 10 mM imidazole had been added (red line). The region between 500 and 600 nm is shown in an enlarged form in the insets. The wavelengths of the absorption maxima are indicated. For LcpK30 R164A, a weak band around 568 nm occurred that was less pronounced (at 570 nm) for the LcpK30 wild type or for other heme-containing muteins.