TABLE 3.

Properties of RoxA and of Lcp proteins

Characteristic	Data according to source/proteina
	Xanth. sp./RoxAb	G. p. VH2/LcpVH2c	R. r. RPK1/LcpRrd	Wt/LcpK30	R164A/LcpK30	T168A/LcpK30	H198A/LcpK30	H203A/LcpK30	H232A/LcpK30	H259A/LcpK30	H266A/LcpK30	R195A/LcpK30	R202A/LcpK30	R328A/LcpK30
Type				Wt	DUF2236 residue	DUF2236 residue	DUF2236 heme ligand	Wt like	Wt like	Wt like	Wt like	Instable mutein	Instable mutein	Wt like
Color	Red	Orange	Brown	Red	Red	Red	Colorless	Red	Red	Red	Red	Colorless	Colorless	Red
Conservation (%)e					99.8	99.6	100	86.5	61.0	64.8	58.2	100	100	100
Yield (purified) (mg/liter LB medium)			3	10	3	4	0.5	1	2	0.3	4	0.1	0.05	0.3
UV/vis spectra as-isolated (nm)
Soret band	407		407	412	412	412		412	412	412	412		411	412
Q band (β)	534		535	544	544	544		544	544	544	544			544
			645
Dithionite reduced
Soret band	417		428 no increase	430 increased	430 no increase	430 increased		430 no increase	430 no increase	430 no increase	430 no increase			430 no increase
Q band (β)	521		531	532	532	532		532	532	532	532			532
Q band (α)	549/553		560	562	562	561		562	562	562	561			562
Reduced + imidazole			428 increased	No effect	No effect	430 increased		Wt like	Wt like	Wt like	Wt like			Wt like
Activity			532 + 561 increase			532 + 562 increase
HPLC product assay	ODTD	Typical pattern	Typical pattern	Typical pattern Wt	Inactive	Residual activity	Inactive	Wt like	Wt like	Wt like	Wt like	Inactive	Residual activity	Wt like
Oxygen consumption (U/mg) (22°C)		1.3	0.9	1.5	<0.1	<0.1	<0.1	Wt like	Wt like	Wt like	Wt like	Inactive <0.1	Inactive <0.1	Wt like
CD spectra				Wt	Wt like	Wt like	Wt like	Wt like			Wt like
Melting point	54.3			61.5				61.6

^aEmpty space indicates that the value has not been determined. Xanth. sp., Xanthomonas sp. 35Y; G. p. VH2, Gordonia polyisoprenivorans strain VH2; R. r. RPK1, Rhodococcus rhodochrous strain RPK1.

^bData from reference 5.

^cData from reference 14.

^dData from reference 15.

^eIn an alignment of 495 Lcp_K30 homologues.