FIG 3.

Effects of pH (A) and temperature (B) on enzyme activity. (A) The optimal pH was determined at 37°C by incubating the enzyme (0.207 nmol) at different pH values in universal buffer (40 mM NH₄HCO₃, 20 mM Na₂HPO₄, 20 mM KH₂PO₄, 20 mM TEA) with the A₅-A₄ chitin oligomer mixture (250 μg) as a substrate for 1 h. (B) For optimum temperature, the enzyme (0.207 nmol) was subjected to incubation at different temperatures in 50 mM TEA buffer (pH 8.0) with the chitin oligomer mixture (250 μg) as a substrate for 1 h. Data given represent the mean ± SD values of the results from three independent experiments.