FIG 5.

UHPLC-ELSD-ESI-MS analysis of chitin tetramer (A₄) (1 mg · ml⁻¹) treated with PgtCDA (0.6 nmol). The ELSD chromatogram shows the elution of initial buffer peaks (2 to 6 min) followed by target peaks. The m/z ratios in the MS spectra correspond to the mass of the substrate (A₄; m/z 831.24), which after incubation with enzyme gives rise to single- and double-deacetylated products A₃D₁ (m/z 789.25) and A₂D₂ (m/z 747.23), respectively. A combined spectrum is also attached for an overview. See the supplemental material for the same analysis of chitin pentamer (A₅) and hexamer (A₆) treated with PgtCDA. intens., intensity; Arb., arbitrary units.