TABLE 1.
Different bacterial expression strains and plasmids used in the expression study to determine the strain yielding recombinant PgtCDA with the highest yield and highest specific activity
| Construct no. | Expression strain | Plasmid | Protein yield (nmol/ml) | Enzyme activity (nkat/ml) | Specific activity (nkat/nmol) |
|---|---|---|---|---|---|
| CDA1 | Rosetta disulfide/pLysSRARE/pCDFDuet1-DsbC/TrxA | pET22b::PgtCDA-Strep IIC | 3.08 | 2 | 1 |
| CDA2 | Rosetta2(DE3)/pLysSRARE | pET22b::PgtCDA-Strep IIC | 2.26 | 58 | 25 |
| CDA3 | BL21(DE3) | pET22b::PgtCDA-Strep IIC | 2.45 | 202 | 82 |
| CDA4 | Rosetta disulfide/pLysSRARE/pCDFDuet1-DsbC/TrxA | pET22b::MBP-PgtCDA-Strep IIC | 4.38 | 719 | 164 |
| CDA5 | Rosetta2(DE3)/pLysSRARE | pET22b::MBP- PgtCDA-Strep IIC | 4.85 | 1,001 | 206 |
| CDA6 | BL21(DE3) | pET22b::MBP-PgtCDA-Strep IIC | 6.57 | 1,700 | 258 |
| CDA7 | ER2508 | pMAL-p5x::MBP-PgtCDA-Strep IIC | 1.70 | 697 | 410 |
| CDA8 | Rosetta2(DE3)/pLysSRARE | pMAL-p5x::MBP-PgtCDA-Strep IIC | 3.30 | 1,485 | 450 |