FIG 4.

Growth of L. pneumophila ST1 (strain LP25) and ST62 (strain LP3) in the biofilm on pPVC (A and C) and water (B and D) in the columns operated at 38°C (○) and in the columns operated at the indicated temperatures (●). The columns were operated at 25°C from day 0 to day 9. The L. pneumophila concentration determined by qPCR (number of gene copies per square centimeter) is shown for the columns operated at 38°C (△) and the columns operated at the other indicated temperatures (▲). Colony counts below the detection level in water (3.3 CFU ml⁻¹) are shown as 1 CFU ml⁻¹.