FIG 2.

Primer coverage for the three available but-targeting primer sets. (A) Flint et al. (2). (B) Vital et al. (3). (C) funbut primers. Maximum likelihood trees of full-length protein coding sequences from the FunGene but database, with the tips of each branch colored to reflect the total number of mismatches each primer set has to that particular sequence (red to green). The clade containing all verified but genes is shaded gray. Sequences with confirmed activity are marked with a blue triangle, and but paralogues are marked with yellow triangles.