TABLE 1.

Primers used in cloning each but-like gene as well as for generating full-length gene amplicons for use in qPCR amplification preference assay^a

Gene	Forward primer (5′ to 3′)	Reverse primer (5′ to 3′)	Annealing temp (°C)	PCR product length (bp)
Butyricicoccus #1	CACCATGAGTATTTTTACAGAATACAGAAGCAAACTGCG	CAATATCGTTTGTTAGAGCGCCGC	53	1,338
Butyricicoccus #2	CACCATGGATTATCAGGCGCTCTATCAG	TTATCTGCGGTTGGAGGTTCTCCA	55	1,344
Megasphaera #1	CACCATGTACAAACAGAAACTGATGA	CCGTTTATTGCTATATCTCCAGATTTTCAT	50	1,323
Megasphaera #2	CACCATGTACAAACTTTCACAAATTCC	TTACATGGAAATCTTGCTGGTCTT	50	1,347
499	CACCATGGATTTTAATCAGGAATACCAGC	TTATTTATTGCTTCTTCTCCAAATATGCAT	53	1,341
35-6-1	CACCATGGATTATTCTAAAGAATATCAA	TTATCTCTTATTAGTTCTTCTCCAAATATTC	48	1,344
494a	CACCATGTCATTTACTCAAGAATATCAGA	TTATTTGTTAGATCTTCTCCAGATGTTAGC	53	1,341
831b	CACCATGGATTTTTCAACTGAATACAAAC	TTATTTGTTGCTTCTTCTCCAGATGTGCAT	53	1,341
992a	CACCATGAGTTACGCAAACGAATATCAA	TTACTTATTGCTGCGTCTCCAGATATGAG	54	1,341
27-5-10	CACCATGGAGCAAACAGAACTTTACCGG	TTACTTTCGGTTGCTTCTGCGCC	55	1,344
68-3-10 #1	CACCATGAGTAAGGATTTTGGATTGT	TTATTTCATCACCTTGCTGCTCG	50	1,383
68-3-10 #2	CACCATGTACAAGGTTTCGACTTTAG	CTAGAACGATGTTTTACTGGTGTATTTCC	50	1,359

^aSee Fig. 3.