TABLE 2.
But enzyme kinetics and amino acid analysesa
| Strain of origin | Closest related species (% 16S BLAST identity) | Gene name | Length (aa)b | Crude activity (μM/min · mg) |
Similarity to Roseburia query sequence (aa)c |
||
|---|---|---|---|---|---|---|---|
| Butyryl-CoA | Propionyl-CoA | % ID | % POS | ||||
| 68-3-10 | Eubacterium nodatum (93) | 68-3-10 #1 | 460 | 77.3 | 406.4 | 47 | 66 |
| 68-3-10 | E. nodatum (93) | 68-3-10 #2 | 452 | 218.1 | 335.4 | 48 | 69 |
| 1161 | Megasphaera elsdenii LC-1 (99) | Megasphaera #1 | 441 | 655.5 | 637.2 | 55 | 69 |
| 1161 | M. elsdenii LC-1 (99) | Megasphaera #2 | 448 | 23,515.6 | 20,747.3 | 49 | 69 |
| BB10 | Butyricicoccus pullicaecorum (94) | Butyricicoccus #1 | 445 | 231.2 | 785.4 | 48 | 67 |
| BB10 | B. pullicaecorum (94) | Butyricicoccus #2 | 447 | 13,367.9 | 12,665.3 | 73 | 85 |
| 35-6-1 | Peptoniphilus grossensis (97) | 36-5-1 | 447 | 18,343.5 | 13,495.2 | 62 | 76 |
| 27-5-10 | Intestinimonas butyriciproducens (99) | 27-5-10 | 447 | 7,004.6 | 5,725.3 | 72 | 85 |
| 494a | Anaerostipes butyraticus (96) | 494a | 446 | 13,692.3 | 12,767.3 | 74 | 85 |
| 992a | Anaerostipes hadrus (95) | 992a | 446 | 16,152.8 | 12,193.9 | 77 | 85 |
| 499 | Roseburia hominis (96) | 499 | 446 | 8,276.8 | 7,946.9 | 83 | 91 |
| 831b | R. hominis (97) | 831b | 446 | 27,819.8 | 27,892.9 | 86 | 91 |
a
Sequences with strong activity are shown in bold.
b
aa, amino acids.
c
ID, identity; POS, positive.