Skip to main content
. 2016 Sep 27;8(10):268. doi: 10.3390/v8100268

Figure 2.

Figure 2

Alignment of amino acid sequences of viral RNA-dependent RNA polymerase (RdRp), and phylogenetic analysis of virus CgCV1. (A) Multiple alignments of putative RdRps of CgCV1 and other chrysoviruses sharing significant sequence similarities with CgCV1. The alignment was performed by CLUSTAL X and highlighted with the GeneDoc application. The conserved motifs of RdRp in dsRNA viruses are indicated by Roman numerals; (B) Phylogenetic analysis of the viral RdRp amino acid sequences of CgCV1. The phylogenic tree was constructed using the neighbor-joining method (1000 bootstrap replicates) in MEGA 6. Members in the families of Totiviridae, Partitiviridae, and Chrysoviridae were selected to conduct the multiple amino acid sequences alignment and phylogenic tree construction. These virus names and GenBank accession numbers are listed as follows: Amasya cherry disease-associated chrysovirus (CAH03664.1), Aspergillus fumigatus chrysovirus (CAX48749.1), Aspergillus ochraceous virus (EU118277), Atkinsonella hypoxylon virus (L39125.1), Beauveria bassiana RNA virus 1 (CCC42235.1), Beet cryptic virus 1 (EU489061.1), Botryotinia fuckeliana partitivirus 1(AM491609), Botryotinia fuckeliana totivirus 1 (AM491608), Brassica campestris chrysovirus 1(AKU48197.1), Ceratocystis resinifera partitivirus (AY603052.1), Colletotrichum acutatum RNA virus 1 (AGL42312.1), Coniothyrium minitans RNA virus (AF527633), Cryphonectria nitschkei chrysovirus 1(ACT79256.1), Fragaria chiloensis cryptic virus (AAZ06131.2), Fusarium oxysporum chrysovirus 1 (ABQ53134.1), Gremmeniella abietina RNA virus MS1 (AY089993), Helminthosporium victoriae 145 S virus (YP_052858.1), Helminthosporium victoriae virus 190 S (U41345), Leishmania RNA virus 1–4 (NP_619653.1), Leishmania RNA virus 2–1 (U32108), Magnaporthe oryzae virus 1 (YP_122352.1), Magnaporthe oryzae chrysovirus 1 (BAJ15133.1), Penicillium chrysogenum virus (YP_392482.1), Raphanus sativas chrysovirus 1(AFE83590.1), Raphanus sativus cryptic virus 2 (ABB04855.1), Saccharomyces cerevisiae virus L-A (AAA50321.1), Saccharomyces cerevisiae virus L-BC (La) (NP_042581.1), Ustilaginoidea virens partitivirus 2 (AGR45851), Ustilaginoidea virens partitivirus 4 (AGJ03719), Ustilaginoidea virens RNA virus 3 (AHH25155.1), Verticillium dahliae chrysovirus 1 (ADG21213.1), Verticillium dahliae partitivirus 1 (AGI52210.1), and White clover cryptic virus 1 (AY705784.1).