Figure 1.

Analysing US3 expression by western blot. Cells were either infected with parental, mutant, recombinant, or revertant viruses. Cell lysates were prepared with radioimmunoprecipitation assay (RIPA) buffer. The pUS3 (A) and gB (B) proteins were separated by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The expression of pUS3 and gB (black arrows) were detected with anti-US3 and anti-gB antibodies, respectively. Heat shock protein 90 α/β (HSP90α/β, 1:1000 dilution) was used as a loading control