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. 2016 Oct 12;8(10):275. doi: 10.3390/v8100275

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Rearrangement of actin cytoskeleton in infected ED cells. (A) ED cells were infected with parental, US3_1-deleted, EHV-1^D207A, US3_4-recombinant, or revertant viruses and imaged by immunofluorescence microscopy using a Zeiss Axiovert fluorescence microscope. Pictures were taken with a 63x oil objective. The nucleus was stained with 4′,6-diamidino-2-phenylindole (DAPI, blue), the actin cytoskeleton was stained with phalloidin-Alexa 568 (red), and the virus-infected cells were visualized through enhanced GFP (eGFP) expression (green). (B and C) A total of 200 infected cells for each virus in three independent experiments were inspected and the percentage of infected cells with or without changes in actin cytoskeleton was calculated. A significant change in actin cytoskeleton rearrangement was detected for either EHV-1ΔUS3 or EHV-1^D207A when compared to parental, recombinant, or revertant viruses (one-way ANOVA; p < 0.05).