Figure 5.

Accumulation of MAPV RNA in cellular fractions of infected deer mouse PMVEC. Vero E6 (circles) and deer mouse PMVEC (squares) were infected with MAPV to examine viral RNA in cells (hatched lines) and supernatants (solid lines) by real-time PCR. Cells were inoculated with 0.1 MOI of MAPV for 1 h, followed by removal of inoculum, 1× wash in PBS and addition of 2% FBS Ham’s F12 medium. The day 0 samples were collected 1 h later. The remaining samples were collected on days 3, 6, 9 and 12 dpi. RNA copies were determined relative to tissue culture infectious dose (TCID₅₀) equivalents.