Figure 1.

Experimental schedule. Seven-week-old female BALB/c mice were sensitized on days 0 and 14 by an intraperitoneal injection of OVA combined with 20 mg of aluminum hydroxide. From days 3–17, the mice were treated two times a week with PBS or bvPLA2 via i.t. injection, and then mice were challenged three times a week with an i.t. injection of OVA (1%) or PBS (days 22–31). Twenty-four hours after the OVA challenge, AHR was measured, and the mice were sacrificed on day 33.