Effects of bvPLA2 on the influx of inflammatory cells in the BAL fluid of OVA-challenged mice. On day 33, the mice were sacrificed and BAL fluid was collected. The total cells and each inflammatory cell (eosinophils, neutrophils, macrophages and lymphocytes) were examined by counting a maximum of 500 cells on a smear prepared by using cytocentrifugation and Diff-Quick staining. The results are expressed as the number of each cell population in 1 mL of BAL fluid. The number of (a) total cells; (b) eosinophils; (c) neutrophils; (d) macrophages; and (e) lymphocytes. The statistical analyses were conducted by one-way ANOVA followed by Newman-Keuls multiple comparison test (*** p < 0.001, ** p <0.01 and * p <0.05 vs. NC group, ###
p < 0.001, ##
p < 0.01 and #
p < 0.05 vs. OVA+PLA2 (10 µg/kg) group; n = 5).