Figure 2.

cDNA sequence and deduced amino acid sequence of BlatPII-c. The deduced amino acid sequence of the mature protein is depicted in bold letters and is preceded by the pro-domain, which contains the sequence PKMCGVT characteristic of the Cys switch (highlighted in green). Sequences corresponding to signal peptide, pro-domain, metalloproteinase domain, spacer region and the disintegrin domain are indicated. The sequences corresponding to the mutated zinc-binding motif and the sequence CIM (Cys-Ile-Met) of the Met-turn are highlighted in yellow, as well as the sequence RGD in the disintegrin domain. Cysteine residues proposed to be related to the lack of proteolytic processing of the disintegrin from the metalloproteinase domain are labeled with asterisks. Primers used to PCR-amplify the cDNA clone and the C-terminal amino acid sequence gathered by MS/MS analysis of a tryptic peptide are shown.