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. 2016 Oct 31;7:436. doi: 10.3389/fimmu.2016.00436

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Copyright © 2016 Wei, Hermosilla, Taubert, He, Wang, Gong, Li, Yang and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Visualization of DNA decorated with histones (H3), neutrophil elastase (NE), and myeloperoxidase (MPO) in N. caninum tachyzoite-induced NETs structures. PMN were stimulated with Neospora caninum (ratio: 1:1, 90 min). DNA decorated with of H3, NE, and MPO within NETs were detected using a scanning confocal microscope. (A) Histone (Green). (D) MPO (Green). (G) NE (Green). (B,E,H) DNA (Red). (C,F,I) Respective merge of DNA decorated with H3, NE, and MPO.