Fig. 1.

Illustration of the effect of image processing on 4D movies. Shown are the first frames from Video 1 and Video 2, which are 4D movies from the same data set before and after deconvolution, respectively. Yeast cells expressing GFP-Vrg4 as an early Golgi marker and Sec7-DsRed as a late Golgi marker were imaged by confocal microscopy, with cell images in the blue channel. Z-stacks of 24 optical sections each were collected every 2 sec. Where indicated, the red and green channels were deconvolved. The movie frames are average projections of the Z-stacks. In the deconvolved movie, maturation events can be observed when green Golgi cisternae turn red [5].