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. 2016 Oct 31;6:36114. doi: 10.1038/srep36114

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Cell viability was determined by SRB assay after 24 h treatment with different concentrations of JB (20, 40, 60, 80 and 100 μM). (b) Morphologic measurements in B16F10 cells were carried out via Hoechst fluorescence staining. (c) Representative images showing the apoptotic cells after treatment with the indicated concentrations of JB. (d) The apoptotic rates of B16F10 cells after JB treatment. The data represent mean ± s.d. of the three independent experiments. *P < 0.05, **P < 0.01 compared with the control group.