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. 2016 Oct 31;6:36103. doi: 10.1038/srep36103

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Copyright © 2016, The Author(s)

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Proteins in whole cell lysate, apical membrane and COM crystal-bound fractions were resolved by 12% SDS-PAGE and subjected to Western blot analysis using rabbit polyclonal anti-α-enolase (Santa Cruz Biotechnology) as a primary antibody. Coomassie Brilliant Blue G-250-stained gel of the COM-bound fraction was also aligned with the immunoblot.