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. 2016 Oct 31;6:36103. doi: 10.1038/srep36103

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Copyright © 2016, The Author(s)

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Purified α-enolase or carbonic anhydrase II with an equal amount (5 μg) was directly incubated with COM crystals at 4 °C overnight. After washing, COM-bound protein was detected by immunofluorescence staining. (A) An overview of the immunofluorescence staining (original magnification power = 200X). (B) Zoom-in images of the immunofluorescence stained protein (shown in red in lower panels) comparing to the bright field (upper panels) (original magnification power = 1,000X).