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. 2016 Oct 31;6:36103. doi: 10.1038/srep36103

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A,B) Cell-COM crystal adhesion assay (original magnification = 400X). The adherent crystals were counted from at least 15 random high power fields (HPFs). (C,D) Crystal-protein binding assay (original magnification = 1,000X). Quantitative analysis of immunofluorescence staining of α-enolase bound on COM crystals in each group was performed using ImageJ software from at least 100 individual COM crystals per group. Each bar represents mean ± SEM of the data obtained from 3 independent experiments. *p < 0.05 vs. uncoated-COM crystal.