Figure 7. Binding of α-enolase on differential crystal types.

Purified α-enolase (5 μg) was directly incubated with COM, COD, brushite, HAP or uric acid crystals using an equal amount of crystals (5 mg/ml artificial urine) at 4 °C overnight. (A) After washing, crystal-bound α-enolase was detected by immunofluorescence staining (shown in red) (original magnification power = 1,000X). (B) Quantitative analysis of immunofluorescence stained α-enolase bound on each crystal type was performed using ImageJ software from at least 100 individual crystals per group. Each bar represents mean ± SEM of the data obtained from 3 independent experiments. *p < 0.05 vs. other four crystal types.