Figure 1. Portion of CD8⁺ T cells reactive to the pool of OLPs covering the whole length of mouse MOG was Qa-1^b restricted.

(A) A schematic view of the mouse MOG OLP library. 15 mer peptides across the whole length of mouse MOG (247aa) and overlapped by 11aa were synthesized. A pool of the 59 OLPs (MOG_pool) at a concentration of 4.2 μg/ml for each peptide was then generated for stimulating CD8⁺ T cells purified from K^b−/−D^b−/− mice. (B) Experimental design for “C”: CD8⁺ T cells purified from K^b−/−D^b−/− mice were stimulated with the MOG_pool in vitro on a weekly basis. Before being stimulated each time, the CD8⁺ T cells were monitored for response to the MOG_pool, using IFN-γ Enzyme-linked ImmunoSpot, in the presence of either C1R or C1R.Qa-1^b cells as antigen presenting cells. (C) Enzyme-linked ImmunoSpot data on day 7 were shown and were representative of three independent experiments. The number at upper left corner of each well is the absolute number of IFN-γ spot forming cells (SFCs) in the corresponding well (50,000 CD8⁺ T cells/well).