Figure 3. Fine mapping of the minimal and optimal Qa-1^b epitope in OLP105.

Progressively N- and C-terminally truncated OLP105 peptides were synthesized and interrogated for their ability to stimulate, using IFN-γ Enzyme-linked ImmunoSpot, an OLP105-reactive CD8⁺ T cell line in the presence of C1R. Qa-1^b cells. IDs and sequences of the truncated peptides were shown in the left and middle panels respectively. Corresponding bars in the right panel showed IFN-γ spot-forming cells (SFCs) per 10⁶ CD8⁺ T cells in response to corresponding peptides. Highlighted bar and sequence displayed the highest response and stimulating peptide sequence respectively, demonstrating that sequence of the optimal and minimal epitope in OLP105 was IICYNWLHR, i.e. MOG_196-204 (or MOG₁₉₆). The data was representative of four independent experiments.