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. 2016 Oct 31;6:36086. doi: 10.1038/srep36086

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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HCT-116 (A) and U-251 MG cells (B) were confined to the central microchamber of the device in hydrogel. An apoptosis sensing dye was perfused into the device via the lateral microchannels. For both cell lines, apoptotic levels were found to increase with time. The graphs show the apoptotic profiles across the delimited region. Position of the pillars is delimited by a grey dashed line. (C,D) For detection and monitoring of ROS production, a commercially available agent that is oxidised by ROS to a fluorescent product was perfused through the system. The graphs show quantification of ROS along the delimited region. Scale bar is 200 μm.