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. 2016 Oct 31;6:36170. doi: 10.1038/srep36170

Figure 5. Effect of marine terpenoids on Topo II α activity.

Figure 5

(A) Effect of compounds 1–3 on topo II activity. Lanes 1–5: 3 (0.08, 0.3125, 1.25, 5, and 20 μg/mL); Lanes 6–10: 1 (0.08, 0.3125, 1.25, 5, and 20 μg/mL); Lanes 11–15: 2 (0.08, 0.3125, 1.25, 5, and 20 μg/mL); Lane 16: positive control, etoposide (500 μM), as topo II poison (induction of linear DNA); Lane 17: plasmid DNA + topo II + solvent control (induction of DNA relaxation); Lane 18: Linear DNA; Lane 19: negative control plasmid DNA (supercoiled DNA); The full length gel of Topo IIα is supplied in Supplementary data Fig. S20 (B) The treatment with marine terpenoids induced the expression of γH2AX protein in Molt 4 cells. The cells were treated with compounds 1-3 (0, 0.0625, 0.125 and 0.25 μg/mL) for 24 h, respectively. Protein expression was analyzed with Western blotting. GAPDH was used the loading control.