Table S1.
Strains used in this study
| Description | Strain | Strain background | Genotype |
| LOH assay diploids | |||
| WT | AZ88 | S288c/RM11 | MATa/α LEU2/leu2∆his3/HIS3 ura3/ura3-Δ0 TRP1/TRP1 lys2/LYS2 HO::KanMX BUD5/bud5Δ::URA3 |
| rnh1∆/∆ rnh201∆/∆ | AZ92 | S288c/RM11 | MATa/α rnh1∆::HYG/rnh1∆::HYG rnh201∆::NAT/rnh201∆::NAT LEU2/leu2∆ his3/HIS3 ura3/ura3-Δ0 TRP1/TRP1 lys2/LYS2 HO::KanMX BUD5/bud5Δ::URA3 |
| RNH1+/∆ rnh201∆/∆ | AZ174 | S288c/RM11 | MATa/α RNH1:HIS3/rnh1∆::HYG rnh201∆::NAT/rnh201∆::NAT LEU2/leu2∆ his3/HIS3 ura3/ura3-Δ0 TRP1/TRP1 lys2/LYS2 HO::KanMX BUD5/bud5Δ::URA3 |
| rnh1∆/∆ RNH201+/∆ | AZ203a | S288c/RM11 | MATa/α rnh1∆::HYG/rnh1∆::HYG RNH201:HIS3/rnh201∆::NAT LEU2/leu2∆ his3/HIS3 ura3/ura3-Δ0 TRP1/TRP1 lys2/LYS2 HO::KanMX BUD5/bud5Δ::URA3 |
| rnh1∆/∆ RNH201 h/∆ | AZ204a | S288c/RM11 | MATa/α rnh1∆::HYG/rnh1∆::HYG RNH201-P45D-Y219A:HIS3/rnh201∆::NAT LEU2/leu2∆ his3/HIS3 ura3/ura3-Δ0 TRP1/TRP1 lys2/LYS2 HO::KanMX BUD5/bud5Δ::URA3 |
| Hotspot hybrid deletion diploids | |||
| rnh1∆/∆ rnh201∆/∆ pgk1∆ | AZ207a | S288c/RM11 | MATa/α PGK1/pgk1∆::HIS3 rnh1∆::HYG/rnh1∆::HYG rnh201∆::NAT/rnh201∆::NAT LEU2/leu2∆ his3/HIS3 ura3/ura3-Δ0 TRP1/TRP1 lys2/LYS2 HO::KanMX BUD5/bud5Δ::URA3 |
| rnh1∆/∆ rnh201∆/∆ snRNA/δ∆ | AZ217a | S288c/RM11 | MATa/α SNR33-YCRCdelta7/snr33-ycrcdelta7∆::HIS3 rnh1∆::HYG/rnh1∆::HYG rnh201∆::NAT/rnh201∆::NAT LEU2/leu2∆ his3/HIS3 ura3/ura3-Δ0 TRP1/TRP1 lys2/LYS2 HO::KanMX BUD5/bud5Δ::URA3 |
| rnh1∆/∆ rnh201∆/∆ pgk1∆ snRNA/δ∆ | AZ221a | S288c/RM11 | MATa/α PGK1/pgk1∆::TRP1 SNR33-YCRCdelta7/snr33-ycrcdelta7∆::HIS3 rnh1∆::HYG/rnh1∆::HYG rnh201∆::NAT/rnh201∆::NAT LEU2/leu2∆ his3/HIS3 ura3/ura3-Δ0 TRP1/trp1∆::KanMX lys2/LYS2 HO::KanMX BUD5/bud5Δ::URA3 |
| Haploids for DRIP | |||
| WT | AZ61c | S288c | MATa LEU2 bud5Δ::URA3 his3 |
| rnh1∆ rnh201∆ | AZ62d | S288c | MATa rnh1∆::HYG rnh201∆::NAT LEU2 bud5Δ::URA3 his3 ade- |
| RNase H1 ChIP | |||
| RNH1-V5 | JA151 | S288c | Mata RNH1-P85-3xV5 MIF2-3xV5:G418 his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 |
| RNH1-V5 rnh201∆ | JA183 | S288c | Mata RNH1-P85-3xV5 rnh201∆::HYG his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 |
| RNH1-V5 Cat Dead | JA172 | S288c | Mata RNH1-P85-3xV5-D193N MIF2-3xV5:G418 his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 |
| RNH1-V5 Cat Dead rnh201∆ | JA184 | S288c | Mata RNH1-P85-3xV5-D193N rnh201∆::HYG his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 |
| WT, No Tag | LW6836a | S288c | MATa leu2 his3 ura3 TRP1 ade- |
| RNH1-HA | AZ205c | RM11 | MATα RNH1-F95-3HA lys2 ura3-Δ0 HO::KanMX leu2 |
| RNase H2 ChIP | |||
| RNH201-HA | AZ218b | S288c | MATa RNH201-6HA:HIS3MX LEU2 bud5Δ::URA3 |