Fig. 2.

Notch3 is differentially up-regulated during CC development. (A) PCR Notch pathway array in rats after 600 mg/L TAA for 8–10 wk (inflamed) (n = 6) vs. control (Left) and 24–26 wk (adenocarcinoma) (n = 6) vs. control (n = 6) (Right). Red labels, at least fourfold up-regulation; green, at leat fourfold down-regulation. (B) qRT-PCR of Notch expression in TAA rat liver normalized to uninjured controls at 8–10 (inflamed), 12–14 (fibrotic), 20 (early malignant), and 26 wk (late malignant) (n = 3; n = 6 control). (C) IHC of TAA time course. CK19 (DAB). Notch3, green; Jagged1, red; αSMA, green. (Scale bar, 100 µm.)