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. 2016 Oct 11;113(43):12202–12207. doi: 10.1073/pnas.1608679113

Fig. 2.

Fig. 2.

Retinol increases 5hmC and reduces 5mC in nESCs. (A) Global levels of 5mC and 5hmC (%) in serum-grown ESCs following 11 d of reprogramming in 2i/LIF medium either with vitamin A (VitA+) or without vitamin A (VitA−) (± SD; n = 3). (B) Retinyl acetate levels in VitA+/− 2i/LIF media formulations (± SD; n = 3). (C) Global levels of 5mC (Upper) and 5hmC (Lower) in nESCs supplemented with increasing levels of retinol for 72 h (black bars) (± SD; n = 3). (D) Global levels of 5mC (Upper) and 5hmC (Lower) in 2i/LIF-conditioned TET-TKO ESCs that were partially rescued by forced expression of TET2 (dark gray) and were exposed to retinol for 72 h (± SD; n = 3).